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KMID : 0525619970020010034
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1997 Volume.2 No. 1 p.34 ~ p.44
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Antibacterial Effect of Chitosan - oligosaccharides to Escherichia coli Causing Diarrhea of Piglets
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이응호/Lee EH
김희경/김희선/강문일/고홍범/김종렴/Kim HK/Kim HS/Kang MI/Koh HB/Kim JR
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Abstract
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Recently chitosan has been focusing on the role of a potential and natural resource with antibacterial or antifungal effect. The purpose of the study was to confirm antibacterial effect of chitosan-oligosaccharides(OCHT)which was characterized by 96% deacethylation, mean molecular weight of 1,814 and mainly 7~ 14oligosacchardes, against 25 E. coli strains isolated from the feces of piglets with diarrhea. Serogroups of E. coli used included 088:K-, 0147:K88ac, 09:K103, 015:K-, 04:Kl2, 0157:K88ac, 015:K-, 09::K103, 020:K101, 01:K- and 0149:K91, E. coli isolates were cultured and adjusted at 2×107 CFU/ml in nutrient broth before adding OCHT diluted at 0.1, 0.01, 0.001, 0.0001, and 0.00002%, respectively. Until 12 hours after the addition and incubation(37℃) of each diluted OCHT in the E. coli-incubating flask, the optical density under 660nm wavelength was examined by one hour interval and showed significant difference(P<0.001) in antibacterial reaction to E.coli between the treatment of 0.0001 ~O.1% OCHT and the control during the test. From approximately 10 minutes to 12 hours after the treatment of 0.0001 ~O.5% OCHT to E. coli (2x107 CFU/ml) incubating in phosphate buffered saline(pH 6.0, 37℃), there was appeared significant decrease(P<0.001) of the number of E. coli colonies compared with the control. Also no E. coli colonies could find in the medium about 12 hours after the addition of 0.0001 ~O.5% OCHT. Consequently, we confirmed that OCHT used has a strong antibacterial effect to E. coli isolates causing diarrhea of piglets.
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KEYWORD
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